Product Detail
Product NameDEPDC5 (Phospho-Ser993) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationThe antibody was purified from rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
ApplicationsWB
Species ReactivityHM
SpecificityDEPDC5(Phospho-Ser993) Antibody detects endogenous levels of DEPDC5 only
when phosphorylated at Ser993.
Immunogen DescA synthesized peptide derived from human DEPDC5 around the phosphorylation site of Ser993.
ConjugateUnconjugated
Other NamesGATOR complex protein DEPDC5; DEP domain-containing protein 5; KIAA0645
Uniprot
O75140
Gene ID
9681
Sdspage MW180kDa
Concentration1 mg/ml
FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM
NaCl,0.02% sodium azide and 50% glycerol.
StorageStore at --20˚C/1 year
Application Details
Western Blot: 1/500 - 1/2000
As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.
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