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DYKDDDDK-Tag Rabbit Polyclonal Antibody#T503

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Product NameDYKDDDDK-Tag Rabbit Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB

SpecificityThe antibody detects transfected proteins contanining DYKDDDDK tag. The antibody recognizes the DYKDDDDK-tag fused to either the amino- or carboxy-terminus of targeted proteins in transfected cells.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence (DYKDDDDK) derived from Human.

Target NameDYKDDDDK-Tag

ConjugateUnconjugated

Concentration1.0mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

WB 1:1000 - 1:3000

Western Blot analysis using FLAG-tag Polyclonal Antibody against HEK293 cells transfected with vector overexpressing FLAG tag (1) and untransfected (2). Secondary antibody was diluted at 1:20000

The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.

If you have published an article using product T503, please notify us so that we can cite your literature.

et al,Calpain-1 Cleaves and Activates Caspase-7J. , (2009),
PMID: 19617626
et al,Calpain-1 cleaves and activates caspase-7. In J Biol Chem on 2009 Sep 11 by Juliette Gafni, Xin Cong, et al..PMID: 19617626 , (2009),
PMID: 19617626

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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