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Myc-Tag Mouse Monoclonal Antibody#T504

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Product NameMyc-Tag Mouse Monoclonal Antibody

Clone No.9E10

Host SpeciesMouse

ClonalityMonoclonal

IsotypeIgG1

PurificationAntibodies were produced from mice ascites by injecting mice with a monoclonal cell line which was fusioned by mouse spleen and SP2/0 myeloma cell. Spleen cells were isolated from mice by immunizing with synthetic peptide and KLH conjugates.

ApplicationsWB

SpecificityThe mouse mAb detects transfected proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino- or carboxy- terminus of targeted proteins in transfected cells.

Immunogen TypePeptide

Immunogen DescPeptide sequence around aa. 410-419 (EQKLISEEDL) derived from human c-Myc.

Target NameMyc-Tag

ConjugateUnconjugated

Accession NoNCBI Protein: NP_002458.2

Concentration1.0mg/ml

FormulationSupplied in mice ascites

StorageStore at 4°C for short term use. Store at -20°C for long term preservation. Avoid freezing and thawing repeatly.

Application Details

Western blotting: 1:5000~1:10000
 

Western blot analysis of extracts from 293 cells transfected with Myc-CDK2 using Myc-Tag mouse mAb #T504.

Epitope tags are artificial epitopes useful for the labeling and detection of proteins. Epitope tags - short amino acid sequences are 'fused' to the N- or C-terminus of the protein.

Munro, S. and Pelham, H.R. (1984) EMBO J. 3, 3087-3093.

If you have published an article using product T504, please notify us so that we can cite your literature.

et al,PKC蔚 phosphorylates MIIP and promotes colorectal cancer metastasis through inhibition of RelA deacetylation. , (2017 Oct 16),
PMID: 29038521
et al,GlcNAcylation of fumarase maintains tumour growth under glucose deficiency. , (2017),
PMID: 28628081
et al,O-GlcNAcylation of PFKFB3 is required for tumor cell proliferation under hypoxia.In Oncogenesis on 2020 Feb 14 by Lei Y, Chen T, et al..PMID:32060258 , (2020),
PMID: 32060258
et al,O-GlcNAcylation of Fumarase Maintains Tumour Growth Under Glucose Deficiency .In Nat Cell Biol ON 2017 Jul by Ting Wang , Qiujing Yu,et al..PMID: 28628081 , (2017),
PMID: 28628081
et al,PKC蔚 phosphorylates MIIP and promotes colorectal cancer metastasis through inhibition of RelA deacetylation.In Nat Commun on 2017 Oct 16 by Tao Chen, Jingjie Li ,et al..PMID: 29038521 , (2017),
PMID: 29038521

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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