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DYKDDDDK-Tag Mouse Monoclonal Antibody#T510

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Product Detail

Product NameDYKDDDDK-Tag Mouse Monoclonal Antibody

Host SpeciesMouse

ClonalityMonoclonal

PurificationAffinity-chromatography

ApplicationsWB IF/ICC IP

Immunogen TypePeptide-KLH

Immunogen DescA synthetic peptide DYKDDDDK coupled to KLH.

Target NameDYKDDDDK-Tag

ConjugateUnconjugated

FormulationMouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20 °C.Stable for 12 months from date of receipt

Application Details

WB: 1:3000~1:10000

IF/ICC: 1:200
DYKDDDDK-Tag (FLAG tag）Mouse Monoclonal antibody A: HEK 293 cell lysate without transfected B: HEK 293 cell lysate transfected with FLAG-tag protein C: IP (PBS + anti- FLAG mAb 4℃ overnight) D: IP (HEK293 transfected+protein G+anti- FLAG mAb 4℃ overnight) E: IP (HEK293 transfected+RIPA+Protein G 4℃ overnight) F: Multi Tag Recombinant protein (expressed in E.coli) TP=Target Protein HC=Heavy chain LC=Light Chain

 DYKDDDDK-Tag (FLAG tag） Mouse mAb is part of the series of Tag antibodies, the excellent quality in the research. FLAG tag antibody is a highly sensitive and affinity PAB applicable to FLAG-tagged fusion protein detection. FLAG tag antibody can detect FLAG tags in internal, C-terminal, or N-terminal recombinant proteins.

If you have published an article using product T510, please notify us so that we can cite your literature.

et al,A small GTPase鈥憀ike protein fragment of Mycoplasma promotes tumor cell migration and proliferation in vitro via interaction with Rac1 and Stat3.In Mol Med Rep on 2014 Jan by Xiaopeng Hu , Jie Yu et al..PMID:24172987 , (2014),
PMID: 24172987

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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