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Location： Home > Tag Antibodies > mCherry Mouse Polyclonal Antibody

mCherry Mouse Polyclonal Antibody#T513

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Product Detail

Product NamemCherry Mouse Polyclonal Antibody

Host SpeciesMouse

ClonalityPolyclonal

PurificationAffinity purification using immunogen.

ApplicationsWB

SpecificityThe mCherry tag antibody can recognize mCherry and mCherry tag fusion proteins.

Target NamemCherry

ConjugateUnconjugated

Concentration1.0mg/ml

FormulationMouse IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details

Western blotting: 1:5000~1:10000

Western blot analysis of mCherry Recombinant protein, using #T513 diluted at 1) 1:5,000, 2) 1:10,000.

mCherry is a fluorophore (a fluorescent molecule) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cells components. mCherry is a monomeric fluorescent construct with peak absorption/emission at 587nm and 610nm, respectively. It is resistant to photobleaching and is stable. mCherry is sometimes preferred to other fluorophores due to its colour, as well as its photostability compared to other monomeric fluorophores.

If you have published an article using product T513, please notify us so that we can cite your literature.

et al,GABA Cells in the Central Nucleus of the Amygdala Promote Cataplexy.In J Neurosci on 2017 Apr 12 by Matthew B Snow, Jimmy J Fraigne,et al..PMID: 28209737 , (2017),
PMID: 28209737

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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