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mCherry Mouse Monoclonal Antibody#T515

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Product Detail

Product NamemCherry Mouse Monoclonal Antibody

Host SpeciesMouse

ClonalityMonoclonal

PurificationAffinity purification using immunogen.

ApplicationsWB

SpecificityThe mCherry tag antibody can recognize mCherry and mCherry tag fusion proteins.

Target NamemCherry

ConjugateUnconjugated

Concentration1.0mg/ml

FormulationMouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details
Western blotting: 1:5000~1:10000
Western blot analysis of mCherry recombinant protein, using #T515 diluted at 1) 1:5,000 2) 1:10,000.
mCherry is a fluorophore (a fluorescent molecule) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cells components. mCherry is a monomeric fluorescent construct with peak absorption/emission at 587 nm and 610 nm, respectively. It is resistant to photobleaching and is stable. mCherry is sometimes preferred to other fluorophores due to its colour, as well as its photostability compared to other monomeric fluorophores.

If you have published an article using product T515, please notify us so that we can cite your literature.

et al,SALM1 controls synapse development by promotIng F-actIn/PIP2-dependent NeurexIn clusterIng. In EMBO J on 2019 Sep 2 by Brouwer M, Farzana F, et al..PMID:31368584 , (2019),
PMID: 31368584
et al,Reduced dynamin-1 levels in neurons lacking MUNC18-1. In J Cell Sci on 2022 Nov 15 by Hanna C A Lammertse, Alessandro Moro,et al..PMID:36245272 , (2022),
PMID: 36245272
et al,Rabphilin-3A negatively regulates neuropeptide release, through its SNAP25 interaction. In Elife on 2024 Oct 16 by Adlin Abramian, Rein I Hoogstraaten,et al..PMID:39412498 , (2024),
PMID: 39412498

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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