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GFP Mouse Monoclonal Antibody (Plant Specific)#T516

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Product Detail

Product NameGFP Mouse Monoclonal Antibody (Plant Specific)

Host SpeciesMouse

ClonalityMonoclonal

PurificationAffinity purification using immunogen.

ApplicationsWB

SpecificityThe GFP tag antibody can recognize GFP,EGFP and GFP, EGFP tag fusion protein.s in Plants.

Target NameGFP

ConjugateUnconjugated

Concentration1.0mg/ml

FormulationMouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details
Western blotting: 1:5000
Western blot analysis of GFP transfected Plants, using #T516 diluted at 1:5,000 1) GFP Mock (control) 2) GFP+ target protein.
The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. In cell and molecular biology, the GFP gene is frequently used as a reporter of expression.

If you have published an article using product T516, please notify us so that we can cite your literature.

et al,Sorting nexin 27 interacts with Fzd7 and mediates Wnt signalling.In Biosci Rep on 2016 Jan 7 by Lixiang Sun, Xiuyong Hu et al..PMID: 26744382 , (2016),
PMID: 26744382

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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