Product Detail
Product NameFlag-Tag Mouse Monoclonal Antibody
Host SpeciesMouse
ClonalityMonoclonal
PurificationAffinity purification using immunogen.
ApplicationsWB,IF IP
Species ReactivityHu Ms Rt
SpecificityThe Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion?proteins.
Target NameFlag-Tag
ConjugateUnconjugated
Concentration1.0mg/ml
FormulationMouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20˚C
Application Details
Western blotting: 1:5000~1:10000
Immunofluorescence: 1:2000
Immunoprecipitation: 1:5000
1ug Flag fusion protein+ Primary antibody #T519 dilution at 1:10,000.
IF analysis of 293 cells transfected with a Flag-tag protein, using anti-Flag-Tag Mouse mAb #T519 at a 1:2000 dilution (blue DAPI, red anti-Flag).
IP antibody use£º5ug Flag Mouse IgG1 per ml Lysate£¬WB 1:5000
1¡¢untransfected 293 cell lysate
2¡¢transfected 293 cell lysate with Flag-tag fusion protein
3¡¢IP (transfected 293+ normal Mouse IgG+Protein G agarose)
4¡¢IP (transfected 293+anti- Flag mAb+ Protein G agarose)
5¡¢IP (transfected 293+Protein G)
6¡¢Recombinant protein (E.coli).
The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.
If you have published an article using product T519, please notify us so that we can cite your literature.
et al,AdenosIne DeamInase ActIng on RNA 1 Associates with Orf Virus OV20.0 and Enhances Viral Replication. In J Virol on 2019 Mar 21 by Liao GR, Tseng YY, et al..PMID:30651363
, (2019),
PMID:
30651363
et al,Pseudomonas aeruginosa pvdQ Gene Prevents Caco-2 Cells from Obstruction of Quorum-Sensing Signal. In Curr Microbiol on 2011 Jan by Lu Ye, Gaopeng Li, et al..PMID: 20490497
, (2011),
PMID:
20490497
et al,Testing for anti-PBP antibody is not useful in diagnosing autoimmune pancreatitis.In Am J Gastroenterol on 2016 Nov by Jorie Buijs, Djuna L Cahen et al..PMID:
, (2016),
PMID:
27325222
et al,Combined in vitro and in silico analyses of FGFR1 variants: genotype-phenotype study in idiopathic hypogonadotropic hypogonadism. In Clin Genet
on 2020 Oct by Daoqi Wang, Yonghua Niu, et al..PMID: 32666525
, (2020),
PMID:
32666525
et al,Cell adhesion molecule L1 like plays a role in the pathogenesis of idiopathic hypogonadotropic hypogonadism. In J Endocrinol Invest on 2021 Aug by Y Chen, T Sun,
et al..PMID:33453020
, (2021),
PMID:
33453020
et al,A variant NS1 protein from H5N2 avian influenza virus suppresses PKR activation and promotes replication and virulence in mammals. In Emerg Microbes Infect
on 2022 Dec by Yun-Ting Chung, Chih-Ying Kuan, et al..PMID:35979918
, (2022),
PMID:
35979918
et al,Epigenetic Control of Mitochondrial Fission Enables Self-Renewal of Stem-like Tumor Cells in Human Prostate Cancer
, (2019),
PMID:
31130467
Yes
