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Tag Antibodies > V5-Tag Mouse Monoclonal Antibody
V5-Tag Mouse Monoclonal Antibody#T605
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IF analysis of 293 cells transfected with a V5-tag protein, using anti-V5Tag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-V5)
IP antibody use：5ug V5 Mouse IgG1 per ml Lysate，WB 1:5000 Lane 1 : untransfected 293 cell lysate Lane 2 : transfected 293 cell lysate with V5-tag fusion protein Lane 3 : IP(untransfted 293+anti-V5 mAb+Protein G agarose) Lane 4 : IP (transfected 293+ normal Mouse IgG+Protein G agarose) Lane 5 : IP (transfected 293+anti-V5 mAb+ Protein G agarose) Lane 6 : IP (transfected 293+Protein G) Lane 7 : Recombinant protein (E.coli)
All lanes : V5-Tag Mouse mAb at 1/3k dilution Lane 1 : HEK 293 cell lysate transfected without V5-tag protein Lane 2 : HEK 293 cell lysate transfected with V5 -tag protein Lane 3 : IP (HEK 293 cell without transfected + anti- V5 mAb 4℃ overnight) Lane 4 : IP (PBS + anti- V5 mAb 4℃ overnight) Lane 5 : IP (HEK293 transfected+protein G+anti- V5 mAb 4℃ overnight)Lane 6 : IP (HEK293 transfected+RIPA+Protein G 4℃ overnight)Lane 7 : Multi Tag Recombinant protein (expressed in E.coli) TP=Target Protein HC=Heavy chain LC=Light Chain
NOTE
Application
- WBWestern Blotting
- IHCImmunohistochemistry
- IFImmunofluorescence
- ICCImmunocytochemistry
- FCFlow Cytometry
- IPImmunoprecipitation
- EELISA
- DBDot Blotting
- ChIPChromatin Immunoprecipitation
- GICAGold Immunochromatography Assay
- NCNegative Control
Species Reactivity
- HuHuman
- MsMouse
- RtRat
- DmDrosophila melanogaster
- CCaenorhabditis elegans
- MkMonkey
- RbRabbit
- BBovine
- DDog
- PPig
- HmHamster
- ChHmChinese Hamster
- ChkChicken
- ShpSheep
