Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate NKD1 in samples. An antibody specific for NKD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNKD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NKD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NKD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:In the mouse, Nkd is a Dishevelled-binding protein that functions as a negative regulator of the Wnt-beta-catenin-Tcf signaling pathway.
The deduced 470-amino acid protein contains an EF-hand Ca(2+)-binding motif. NKD1 shares about 44% overall identity with NKD2, including more than 75% identity in 4 major homology domains, including the EF-hand motif. Northern blot analysis revealed wide expression of 5 transcripts of 9.3 to 2.3 kb in adult and fetal tissues and in cancer cell lines. Highest expression was detected in fetal kidney, followed by fetal lung, placenta, and adult liver, kidney, pancreas, and spleen. Multiple transcripts were generated through the use of alternative polyadenylation sites.
