• Product NameGLI1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsIHC WB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total GLI1 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human GLI1
• Target NameGLI1
• Alternative Names    GLI
• Accession No. Swiss-Prot#: P08151
  NCBI Gene ID: 2735
• UniprotP08151
• Gene ID2735;
• Calculated MW 118kd
• Concentration 0.5mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1000
  Immunohistochemistry: 1: 20-100

This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene.

• Gel: 6%SDS-PAGE
  Lysate: 40 μg, Lane 1-4: NIH/3T3£¬Raji£¬PC-3 and Hepg2 cell,
  Primary antibody:GLI1 antibody at dilution 1/200 dilution,
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
  Exposure time: 10 seconds
• The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using GLI1 Antibody at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)
• The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using GLI1 Antibody at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting