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PKM2(phospho-Ser37) Antibody #11456

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Catalog No. Package Size Price Quantity
50ul $219.00
100ul $319.00
Availability:Yes
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Description
  • Product NamePKM2(phospho-Ser37) Antibody
  • Host SpeciesRabbit
  • ClonalityPolyclonal
  • PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • ApplicationsWB IF
  • Species ReactivityHu
  • SpecificityThe antibody detects endogenous level of PKM2 only when phosphorylated at serine 37.
  • Immunogen TypePeptide-KLH
  • Immunogen DescriptionPeptide sequence around phosphorylation site of serine 37(I-D-S(p)-P-P) derived from Human PKM2.
  • Target NamePKM2
  • ModificationPhospho
  • Alternative Names    PKM, PK3, OIP3, PK2
  • Accession No. Swiss-Prot#: P14618 NCBI Protein#: NP_872270.1
  • UniprotP14618
  • Gene ID5315;
  • SDS-PAGE MW 60kd
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C
Application Details
Background

Published Papers

ATM inhibition blocks glucose metabolism and amplifies the sensitivity of resistant lung cancer cell lines to oncogene driver inhibitors InCancer MetabOn2023 Nov 6byCristina Terlizzi , Viviana De Rosa et al..PMID:37932830, , (2023),
PMID: 37932830
Warburg-like metabolic transformation underlies neuronal degeneration in sporadic Alzheimer's disease.In Cell Metab. 2022 Sep 6 by Larissa Traxler 1, Joseph R Herdy,et al..PMID: 35987203, , (2022),
PMID: 35987203
LncCCAT1 interaction protein PKM2 upregulates SREBP2 phosphorylation to promote osteosarcoma tumorigenesis by enhancing the Warburg effect and lipogenesis. In Int J Oncol on 2022 Apr by Feifei Pu, Jianxiang Liu,et al..PMID:35244192, , (2022),
PMID: 35244192
Oncogenic TRIB2 interacts with and regulates PKM2 to promote aerobic glycolysis and lung cancer cell procession. In Cell Death Discov on 2022 Jul 5 by Yuan-Rong Liu, Dan-Dan Song, et al..PMID:35790734, , (2022),
PMID: 35790734
View All Published Papers TRIM35-mediated degradation of nuclear PKM2 destabilizes GATA4/6 and induces P53 in cardiomyocytes to promote heart failure. In Sci Transl Med on 2022 Nov 2 by Maria Areli Lorenzana-Carrillo, Keshav Gopal,et al..PMID: 36322626, , (2022),
PMID: 36322626
Phosphoproteome of signaling by ErbB2 in ovarian cancer cells. In Biochim Biophys Acta Proteins Proteom on 2022 Apr 1 by C Sidhanth, S Bindhya,et al..PMID:35158093, , (2022),
PMID: 35158093
Non-Canonical Role of PDK1 as a Negative Regulator of Apoptosis through Macromolecular Complexes Assembly at the ER�CMitochondria Interface in Oncogene-Driven NSCLC. In Cancers (Basel) on 2021 Aug 17 by Viviana De Rosa, Francesca Iommelli,et al..PMID:34439291, , (2021),
PMID: 34439291
Estrogen activates pyruvate kinase M2 and increases the growth of TSC2-deficient cells.In PLoS One on 2020 Feb 20; by Lu Y, Liu X, et al..PMID: 32078667, , (2020),
PMID: 32078667
Exploring rapamycin-induced pro-survival pathways in Tuberous Sclerosis Complex and the development of alternative therapies, , (2020),
PMID:
Nuclear translocation of PKM2/AMPK complex sustains cancer stem cell populations under glucose restriction stress.In Cancer Lett. On 2018 May 1 et al..PMID:29408265 , , (2018),
PMID: 29408265
Cross-Talk Between Alternatively Spliced UGT1A Isoforms and Colon Cancer Cell Metabolism.In Mol Pharmacol on 2017 Mar by Yannick Audet-Delage,Michle Rouleau,et al..PMID: 28049773, , (2017),
PMID: 28049773
Glut1 Promotes Cell Proliferation, Migration and Invasion by Regulating Epidermal Growth Factor Receptor and Integrin Signaling in Triple-Negative Breast Cancer Cells.In BMB Rep on 2017 Mar by Sunhwa Oh, Hyungjoo Kim,et al..PMID: 27931517, , (2017),
PMID: 27931517
Selective GPR55 Antagonism Reduces Chemoresistance in Cancer Cells .In Pharmacol Res on 2016 Sep by Nagendra S Singh , Michel Bernier et al..PMID: 27423937, , (2016),
PMID: 27423937
Vitamin C uncouples the Warburg metabolic switch in KRAS mutant colon cancer.In Oncotarget on 2016 Jul 26 by Oscar Aguilera , Mara Mu?oz-Sagastibelza et al..PMID:27323830 , , (2016),
PMID: 27323830
PKM2 dephosphorylation by Cdc25A promotes the Warburg effect and tumorigenesis.In Nat Commun on 2016 Aug 3 by Ji Liang , Ruixiu Cao et al..PMID: 27485204, , (2016),
PMID: 27485204
ECM1 promotes the Warburg effect through EGF-mediated activation of PKM2.In Cell Signal on 2015 Feb by Kyung-Min Lee, Keesoo Nam et al ..PMID: 25446258, , (2015),
PMID: 25446258
Estradiol-17β Upregulates Pyruvate Kinase M2 Expression to Coactivate Estrogen Receptor-α and to Integrate Metabolic Reprogramming With the Mitogenic Response in Endometrial Cells .In J Clin Endocrinol Metab on 2014 Oct by Salama A Salama, Mahmoud A Mohammad et al..PMID:24471565, , (2014),
PMID: 24471565
ERK1/2-dependent phosphorylation and nuclear translocation of PKM2 promotes the Warburg effect.In Nat Cell Biol on 2012 Dec by Weiwei Yang, Yanhua Zheng,et al..PMID:23178880, , (2012),
PMID: 23178880
Please let us know if you have published research using #11456 so that we can cite your reference.

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Application

WBWestern Blotting

IHCImmunohistochemistry

IFImmunofluorescence

ICCImmunocytochemistry

FCFlow Cytometry

IPImmunoprecipitation

EELISA

DBDot Blotting

ChIPChromatin Immunoprecipitation

GICAGold Immunochromatography Assay

NCNegative Control

Species Reactivity

HuHuman

MsMouse 

RtRat 

Dm Drosophila melanogaster

C Caenorhabditis elegans

MkMonkey

RbRabbit

B Bovine 

D Dog

PPig

HmHamster

ChHm Chinese Hamster 

ChkChicken  

ShpSheep