• Product NameHistone H3 (Ab-27) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total Histone H3 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.26-30(A-R-K-S-A) derived from Human Histone H3.
• Target NameHistone H3
• Alternative Names    H3/A; H3FA
• Accession No. Swiss-Prot: P68431
  NCBI Gene ID: 8350
  NCBI mRNA: NM_003529.2
  NCBI Protein: NP_003520.1
• UniprotP68431
• Gene ID8350;8351;8352;8353;8354;8355;8356;8357;8358;8968;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C

• Predicted MW: 17kd

  Western blotting: 1:500~1:1000

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

• Western blot analysis of extracts from HT29 and C6 cells using Histone H3 (Ab-27) Antibody #21689.
• Western blot analysis of extracts of various cell lines, using Histone H3 (Ab-27) Antibody #21689

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting