• Product NameDRP1 (Phospho-Ser637) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB,IHC,IF,ELISA
• Species ReactivityHu Rt Ms
• SpecificityThe antibody detects endogenous levels of DRP1 only when phosphorylated at serine 637.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Serine637(K-L-S(p)-A-R) derived from Human DRP1.
• Target NameDRP1
• ModificationPhospho
• Alternative Names    DLP1; DNM1L; DRP1; DVLP; Dymple
• Accession No. Swiss-Prot#: O00429;
  NCBI Gene#: 10059;
  NCBI Protein#: NP_036192.2.
• UniprotO00429
• Gene ID10059;
• SDS-PAGE MW 82kd
• Concentration 0.5 mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into membrane-associated tubular structures that wrap around the scission site to constrict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. Through its function in mitochondrial division, ensures the survival of at least some types of postmitotic neurons, including Purkinje cells, by suppressing oxidative damage. Required for normal brain development, including that of cerebellum. Facilitates developmentally regulated apoptosis during neural tube formation.

Shin H.-W., J. Biochem. 122:525-530(1997).
Hong Y.-R., Biochem. Biophys. Res. Commun. 249:697-703(1998).
Imoto M., J. Cell Sci. 111:1341-1349(1998).

• Western blot analysis of extracts from JK cells and K562 cells using DRP1 (Phospho-Ser637) Antibody #11842.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting