• Product NameIKK-b(Ab-199) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total IKK-bprotein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.197~201 (Q-K-Y-T-V) derived from Human IKK-b.
• Target NameIKK-b
• Alternative Names    I-kappa-B kinase 2; I-kappa-B-kinase beta; IKK2; IkBKB; NFKBIKB
• Accession No. Swiss-Prot: O14920
  NCBI Protein: NP_001547.1
• UniprotO14920
• Gene ID3551;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 87kd
  Western blotting: 1:500~1:1000
  Immunofluorescence: 1:100~1:200

Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3.

Chen RA, et al. PLoS Pathog. 2008 Feb 8;4(2):e22
Yen CJ,et al. Cancer Res. 2008 Apr 15;68(8):2632-40

• Western blot analysis of extracts from MDA cells using IKK-b(Ab-199) Antibody #21305.
• Immunofluorescence staining of methanol-fixed Hela cells using IKK-b(Ab-199) Antibody #21305.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting