• Product NameCyclin B1(Ab-147) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total Cyclin B1 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.145~149 (A-F-S-D-V) derived from Human Cyclin B1.
• Target NameCyclin B1
• Accession No. Swiss-Prot: P14635
  NCBI Protein: NP_114172.1
• UniprotP14635
• Gene ID891;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 60kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

The protein encoded by Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

Norbury, C. and Nurse, P. (1992) Annu. Rev. Biochem. 61, 441-470.
Atherton-Fessler, S. et al. (1993) Mol. Cell. Biol. 13, 1675-1685.
Galaktionov, K. et al. (1995) Genes Dev. 9, 1046-1058.

• Western blot analysis of extracts from HepG2 and HT29 cells using Cyclin B1(Ab-147) Antibody #21540.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cyclin B1(Ab-147) Antibody #21540(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence staining of methanol-fixed Hela cells using Cyclin B1(Ab-147) Antibody #21540.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting