• Product NameCD34 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total CD34 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.377~381(Q-H-V-V-A) derived from Human CD34.
• Target NameCD34
• Alternative Names    CD34
• Accession No. Swiss-Prot: P28906
  NCBI Protein: NP_001020280.1
• UniprotP28906
• Gene ID947;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 110kd
  Western blotting: 1:500~1:1000
  

Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

Fackler M.J.et.al(1990)Chem. 265:11056-11061

• Western blot analysis of extract from HUVEC cells and Hematopoietic Stem Cells from Umbilical Cord Blood using CD34 Antibody #21469

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting