• Product NameB4GALNT1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total B4GALNT1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human B4GALNT1.
• Target NameB4GALNT1
• Alternative Names    Beta-1; 4 N-acetylgalactosaminyltransferase 1; (N-acetylneuraminyl)-galactosylglucosylceramide; GM2/GD2 synthase; GalNAc-T
• Accession No. Swiss-Prot: Q00973
  NCBI Gene ID: 2583
• UniprotQ00973
• Gene ID2583;
• SDS-PAGE MW 60kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Involved in the biosynthesis of gangliosides GM2, GD2 and GA2.

Nagata Y., J. Biol. Chem. 267:12082-12089(1992).
Nagata Y., J. Biol. Chem. 269:7045-7045(1994).
Li J., J. Biol. Chem. 275:41476-41486(2000).

• Western blot analysis of extracts from HeLa cells, K562 cells, HepG2 cells and HuvEC cells, using B4GALNT1 antibody #34491.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting