• Product NameBDKRB2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total BDKRB2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to residues near the C terminal of human bradykinin receptor B2
• Target NameBDKRB2
• Alternative Names    B2R; BK2; BK-2; BKR2; BRB2
• Accession No. Swiss-Prot#: P30411
  NCBI Gene ID: 624
  Gene Accssion: NP_000614.1
• UniprotP30411
• Gene ID624;
• SDS-PAGE MW 44kd
• Concentration 1.1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:50-1:200

This gene encodes a receptor for bradykinin. The 9 aa bradykinin peptide elicits many responses including vasodilation, edema, smooth muscle spasm and pain fiber stimulation. This receptor associates with G proteins that stimulate a phosphatidylinositol-calcium second messenger system. Alternate start codons result in two isoforms of the protein.

• Gel: 10%SDS-PAGE
  Lysates (from left to right): Human leiomyosarcoma tissue and NIH/3T3 cell
  Amount of lysate: 50ug per lane
  Primary antibody: 1/500 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 1 minute
• Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue using #36766 at dilution 1/30.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting