• Product NameBECN1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total BECN1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from internal residues of human beclin 1, autophagy related
• Target NameBECN1
• Alternative Names    ATG6; VPS30; beclin1
• Accession No. Swiss-Prot#: Q14457
  NCBI Gene ID: 8678
  Gene Accssion: NP_003757
• UniprotQ14457
• Gene ID8678;
• SDS-PAGE MW 52kd
• Concentration 1.8mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:25-1:100

Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration

• Gel: 8%SDS-PAGE
  Lysates (from left to right): 293T and Raji cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/200 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 1 minute
• Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using #36768 at dilution 1/20.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting