• Product NameEGR4 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total EGR4 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from internal residues of human early growth response 4
• Target NameEGR4
• Alternative Names    NGFIC; NGFI-C; PAT133
• Accession No. Swiss-Prot#: Q05215
  NCBI Gene ID: 1961
  Gene Accssion: NP_001956
• UniprotQ05215
• Gene ID1961;
• SDS-PAGE MW 51kd
• Concentration 0.4mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

Early growth response protein 4 (EGR-4), also known as AT133, is a protein that in humans is encoded by the EGR4 gene. EGR-4 is a member of the early growth response (EGF) family of zinc finger transcription factors. Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-GCGGGGGCG-3' (GSG). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.

• Gel: 8%SDS-PAGE
  Lysates (from left to right): Human fetal brain tissue
  Amount of lysate: 40ug per lane
  Primary antibody: 1/400 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 35 seconds
• Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue using #36837 at dilution 1/25.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting