• Product NameDUSP8 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total DUSP8 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide of human DUSP8
• Target NameDUSP8
• Alternative Names    HB5; HVH8; HVH-5; C11orf81
• Accession No. Swiss-Prot#: Q13202
  NCBI Gene ID: 1850
  Gene Accssion: NP_004411
• UniprotQ13202
• Gene ID1850;
• SDS-PAGE MW 66kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• WB:1000-1:5000
  IHC: 1:50-1:200

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates SAPK/JNK and p38, is expressed predominantly in the adult brain, heart, and skeletal muscle, is localized in the cytoplasm, and is induced by nerve growth factor and insulin.

• Gel: 8%SDS-PAGE Lysate: 40 ug Lane: Mouse brain tissue lysate Primary antibody: at dilution 1/1200 Secondary antibody: (HRP-conjugated Goat anti rabbit IgG) at 1/5000 dilution Exposure time: 10 seconds
• The image on the left is immunohistochemistry of paraffinembedded Human breast cancer tissue using at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: 200)
• The image on the left is immunohistochemistry of paraffinembedded Human gastric cancer tissue using at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: 200)
• Gel: 8%SDS-PAGE Lysate: 40 ug Lane: Mouse brain tissue lysate Primary antibody: at dilution 1/1200 Secondary antibody: (HRP-conjugated Goat anti rabbit IgG) at 1/5000 dilution Exposure time: 10 seconds
• The image on the left is immunohistochemistry of paraffinembedded Human breast cancer tissue using at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: 200)
• The image on the left is immunohistochemistry of paraffinembedded Human gastric cancer tissue using at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: 200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting