• Product NamePGA5 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total PGA5 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from internal residues of human Pepsinogen 5, group I (pepsinogen A)
• Target NamePGA5
• Alternative Names    PGA3; PGA4
• Accession No. Swiss-Prot#: P0DJD9
  NCBI Gene ID: 5222
  Gene Accssion: NP_055039
• UniprotP0DJD9
• Gene ID5222;
• SDS-PAGE MW 42kd
• Concentration 2.1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

The genes coding for human pepsinogen (PGA3, PGA4, and PGA5) were assigned to chromosome region 11q13 by in situ hybridization. Previously we localized the PGA gene complex to a centromeric region of chromosome 11 (p11----q13) by Southern blot analysis of mouse-human somatic cell hybrids.?Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.

• Gel: 10%SDS-PAGE
  Lysates (from left to right): Human liver cancer tissue
  Amount of lysate: 60ug per lane
  Primary antibody: 1/1000 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 10 minutes
• Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue using #37105 at dilution 1/60.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting