• Product NameSMURF2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsIHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total SMURF2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from internal residues of human SMAD specific E3 ubiquitin protein ligase 2
• Target NameSMURF2
• Alternative Names    DKFZp686F0270;SMUF2; SMURF2
• Accession No. Swiss-Prot#: Q9HAU4
  NCBI Gene ID: 64750
  Gene Accssion: NP_073576
• UniprotQ9HAU4
• Gene ID64750;
• Concentration 1.7mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Immunohistochemistry: 1:25-1:100

E3 ubiquitin-protein ligase SMURF2?is an?enzyme?that in humans is encoded by the?SMURF2?gene. E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3.

• Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue using #37124 at dilution 1/15.
• Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using #37124 at dilution 1/15.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting