• Product NameCDC23 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total CDC23 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from internal residues of human cell division cycle 23
• Target NameCDC23
• Alternative Names    APC8; CUT23; ANAPC8
• Accession No. Swiss-Prot#: Q9UJX2
  NCBI Gene ID: 8697
  Gene Accssion: NP_004652
• UniprotQ9UJX2
• Gene ID8697;
• SDS-PAGE MW 69kd
• Concentration 2.9mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:50-1:200

The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins.

• Gel: 6%SDS-PAGE
  Lysates (from left to right): RAW264.7 and hepG2 cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/200 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 40 seconds
• Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using #37165 at dilution 1/70.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting