• Product NameNUAK1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsIHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total NUAK1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from internal residues of human NUAK family, SNF1-like kinase, 1
• Target NameNUAK1
• Alternative Names    ARK5
• Accession No. Swiss-Prot#: O60285
  NCBI Gene ID: 9891
  Gene Accssion: NP_055655
• UniprotO60285
• Gene ID9891;
• Concentration 2.4mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Immunohistochemistry: 1:25-1:100

NUAK family SNF1-like kinase 1?also known as?AMPK-related protein kinase 5?(ARK5) is an?enzyme?that in humans is encoded by the?NUAK1?gene. Serine/threonine-protein kinase involved in various processes such as cell adhesion, regulation of cell ploidy and senescence, cell proliferation and tumor progression. Phosphorylates ATM, CASP6, LATS1, PPP1R12A and p53/TP53. Acts as a regulator of cellular senescence and cellular ploidy by mediating phosphorylation of 'Ser-464' of LATS1, thereby controlling its stability. Controls cell adhesion by regulating activity of the myosin protein phosphatase 1 (PP1) complex. Acts by mediating phosphorylation of PPP1R12A subunit of myosin PP1: phosphorylated PPP1R12A then interacts with 14-3-3, leading to reduced dephosphorylation of myosin MLC2 by myosin PP1.

• Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue using #37345 at dilution 1/60.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting