• Product NameAGO4 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total AGO4 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from internal residues of human argonaute RISC catalytic component 4
• Target NameAGO4
• Alternative Names    EIF2C4
• Accession No. Swiss-Prot#: Q9HCK5
  NCBI Gene ID: 192670
  Gene Accssion: NP_060099
• UniprotQ9HCK5
• Gene ID192670;
• SDS-PAGE MW 97kd
• Concentration 1.8mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  

This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic containing PAZ and PIWI domains, and it may play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a cluster of closely related family members including argonaute 3, and eukaryotic translation initiation factor 2C, 1.

• Gel: 6%SDS-PAGE
  Lysates (from left to right): Raji, 293T and PC3 cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/450 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 1 minute

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting