• Product NamePFKFB2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total PFKFB2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to residues near the N terminal of human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
• Target NamePFKFB2
• Alternative Names    PFK-2/FBPase-2
• Accession No. Swiss-Prot#: O60825
  NCBI Gene ID: 5208
  Gene Accssion: NP_001018063
• UniprotO60825
• Gene ID5208;
• SDS-PAGE MW 58kd
• Concentration 2.3mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  

The protein encoded by this gene is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene.?

• Gel: 8%SDS-PAGE
  Lysate: 40ug NIH/3T3 cell
  Primary antibody: 1/287.5 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 1 minute

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting