• Product NameSMAD3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total SMAD3 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from internal residues of human SMAD family member 3
• Target NameSMAD3
• Alternative Names    LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
• Accession No. Swiss-Prot#: P84022
  NCBI Gene ID: 4088
  Gene Accssion: NP_005893
• UniprotP84022
• Gene ID4088;
• SDS-PAGE MW 48kd
• Concentration 1.5mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

• Gel: 8%SDS-PAGE
  Lysates (from left to right): Human kindey cancer tissue
  Amount of lysate: 40ug per lane
  Primary antibody: 1/400 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 1 minute
• Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue using #37418 at dilution 1/25.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting