• Product NameDGAT1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• Purificationaffinity purified by Protein A
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total DGAT1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionKLH conjugated synthetic peptide derived from human DGAT1
• Target NameDGAT1
• Alternative Names    ARAT; DGAT; ARGP1
• Accession No. Swiss-Prot#: O75907
  NCBI Gene ID: 8694
  Gene Accssion: NP_036211
• UniprotO75907
• Gene ID8694;
• SDS-PAGE MW 55kd
• Concentration 1mg/ml
• Formulation 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
• Storage Store at -20˚C

• WB1:500-2000;
  IHC 1:100-500;

This gene encodes an multipass transmembrane protein that functions as a key metabolic enzyme. The encoded protein catalyzes the conversion of diacylglycerol and fatty acyl CoA to triacylglycerol. This enzyme can also transfer acyl CoA to retinol. Activity of this protein may be associated with obesity and other metabolic diseases

• Sample: Lane 1: Mouse Adipose tissue lysates Lane 2: Mouse Adrenal gland tissue lysates Lane 3: Rat Adipose tissue lysates Lane 4: Rat Stomach tissue lysates Lane 5: Rat Testis tissue lysates Lane 6: Human HL60 cell lysates Lane 7: Human HepG2 cell lysates Primary: Anti-DGAT1 at 1/300 dilution Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 60 kD
• Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DGAT1) Polyclonal Antibody, at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting