• Product NameCASP8AP2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total CASP8AP2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from internal residues of human caspase 8 associated protein 2
• Target NameCASP8AP2
• Alternative Names    CED-4; FLASH; RIP25
• Accession No. Swiss-Prot#: Q9UKL3
  NCBI Gene ID: 9994
  Gene Accssion: NP_036247
• UniprotQ9UKL3
• Gene ID9994;
• SDS-PAGE MW 223kd
• Concentration 1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:25-1:100

This protein is highly similar to FLASH, a mouse apoptotic protein identified by its interaction with the death-effector domain (DED) of caspase 8. Studies of FLASH protein suggested that this protein may be a component of the death-inducing signaling complex that includes Fas receptor, Fas-binding adapter FADD, and caspase 8, and plays a regulatory role in Fas-mediated apoptosis. Alternative splicing results in multiple transcript variants encoding the same protein.

• Gel: 6%SDS-PAGE
  Lysate: 40ug Hela cell
  Primary antibody: 1/250 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 2 minutes
• Immunohistochemical analysis of paraffin-embedded Human brain tissue using #37574 at dilution 1/20.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting