• Product NameMyoD(Phospho-Ser200) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of MyoD only when phosphorylated at serine 200.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 200 (A-S-S(p)-P-R) derived from Human MyoD.
• Target NameMyoD
• ModificationPhospho
• Alternative Names    MYF3; MYOD; MYOD1
• Accession No. Swiss-Prot: P15172
  NCBI Protein: NP _002469.2
• UniprotP15172
• Gene ID4654;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 40kd
  Western blotting: 1:500~1:1000

MyoD encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis.

Emmanuel G, et al. J. Biol. Chem., Jun 2000; 275: 18767 - 18776
Tintignac LA, et al. Mol Cell Biol. 2004 Feb; 24(4): 1809-1821.
Kitzmann M, et a. Mol Cell Biol. 1999 Apr; 19(4): 3167-3176.

• Western blot analysis of extracts from C2C12 and 3T3 cells untreated or treated with EGF using MyoD (Phospho-Ser200) Antibody #11077.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting