• Product NameMAP1LC3C Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total MAP1LC3C protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to residues near the N terminal of human microtubule-associated protein 1 light chain 3 gamma
• Target NameMAP1LC3C
• Alternative Names    LC3C; ATG8J
• Accession No. Swiss-Prot#: Q9BXW4
  NCBI Gene ID: 440738
  Gene Accssion: NP_001004343
• UniprotQ9BXW4
• Gene ID440738;
• SDS-PAGE MW 17kd
• Concentration 2.6mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  

Autophagy is a highly regulated bulk degradation process that plays an important role in cellular maintenance and development. MAP1LC3C is an ortholog of the yeast autophagosome protein Atg8

• Gel: 10%SDS-PAGE
  Lysates (from left to right): 823 and Raji cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/450 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 2 minutes

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting