• Product NamePIM3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB,IHC,ELISA
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total PIM3 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to residues near the C terminal of human pim-3 oncogene
• Target NamePIM3
• Alternative Names    pim-3
• Accession No. Swiss-Prot#: Q86V86
  NCBI Gene ID: 415116
  Gene Accssion: NP_001001852
• UniprotQ86V86
• Gene ID415116;
• SDS-PAGE MW 36kd
• Concentration 1.2 mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:1000-1:5000
  Immunohistochemistry: 1:50-1:200

The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is overexpressed in hematological and epithelial tumors and is associated with MYC coexpression. It plays a role in the regulation of signal transduction cascades, contributing to both cell proliferation and survival, and provides a selective advantage in tumorigenesis.

• Gel: 8%SDS-PAGELysate: 40 μgLane: Mouse brain tissue lysatePrimary antibody: 37701(PIM3 Antibody) at dilution 1/1350Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilutionExposure time: 5 seconds
• The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 37701(PIM3 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting