• Product NameNDUFAF4 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total NDUFAF4 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to residues near the C terminal of human NADH dehydrogenase (ubiquinone) complex I, assembly factor 4
• Target NameNDUFAF4
• Alternative Names    My013; C6orf66; HRPAP20; HSPC125; bA22L21.1
• Accession No. Swiss-Prot#: Q9P032
  NCBI Gene ID: 29078
  Gene Accssion: NP_054884
• UniprotQ9P032
• Gene ID29078;
• SDS-PAGE MW 20kd
• Concentration 2.8mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:50-1:200

NADH:ubiquinone oxidoreductase (complex I) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. This gene encodes a complex I assembly factor. Mutations in this gene are a cause of mitochondrial complex I deficiency.

• Gel: 10%SDS-PAGE
  Lysate: 40ug Raji cell
  Primary antibody: 1/300 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 5 seconds
• Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue using #37756 at dilution 1/45.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting