• Product NameSmad1/Smad5/Smad8 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total Smad1, Smad5, Smad8 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.461-465(I-S-S-V-S) derived from Human Smad1. This sequence has the same homology in Smad5 and Smad8.
• Target NameSmad1/Smad5/Smad8
• Alternative Names    Mad-related protein 1, SMAD 1, hSMAD1, Transforming growth factor-beta-signaling protein 1,
• Accession No. Swiss-Prot:Q15797
  Gene ID:4086
• UniprotQ15797
• Gene ID4086;
• Concentration 1.0mg/ml

• Predicted MW: 60kd
  Western blotting: 1: 500~1: 1000

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD).SMAD5 is a receptor-regulated SMAD (R-SMAD). SMAD9 is a receptor-regulated SMAD (R-SMAD). Has been shown to be activated by activin type I receptor-like kinases (ALK-2, ALK-3, ALK-6) which stimulate heteromerization between SMAD9 and SMAD4. May play a role in osteoblast differentiation and maturation.

• Western blot analysis of extracts from 3T3 cells using Smad1/Smad5/Smad8 Antibody #21684.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting