• Product NamePARP8 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total PARP8 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to residues near the C terminal of human poly (ADP-ribose) polymerase family, member 8
• Target NamePARP8
• Alternative Names    ARTD16; pART16
• Accession No. Swiss-Prot#: Q8N3A8
  NCBI Gene ID: 79668
  Gene Accssion: NP_078891
• UniprotQ8N3A8
• Gene ID79668;
• SDS-PAGE MW 96kd
• Concentration 0.8 mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:50-1:200

Poly(ADP-ribosylation) is a method of DNA damage-dependent posttranslational modification that helps to rescue injured proliferating cells from cell death. The PARP (poly(ADP-ribose) polymerase) proteins comprise a superfamily of enzymes that functionally modify histones and other nuclear proteins, thereby preventing cell death.?

• Gel: 6%SDS-PAGE Lysate: 40 μg Lane: Mouse testis tissue lysate Primary antibody: PARP8 Antibody at dilution 1/200 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 40 seconds
• The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using PARP8 Antibody at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting