• Product NamePDE4A Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total PDE4A protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from internal residues of human phosphodiesterase 4A, cAMP-specific
• Target NamePDE4A
• Alternative Names    PDE4; DPDE2; PDE46
• Accession No. Swiss-Prot#: P27815
  NCBI Gene ID: 5141
  Gene Accssion: NP_001104777/P27815
• UniprotP27815
• Gene ID5141;
• SDS-PAGE MW 98kd
• Concentration 2.4mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:50-1:200

The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE4 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

• Gel: 6%SDS-PAGE
  Lysates (from left to right): Hela and A172 cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/300 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 20 seconds
• Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using #37809 at dilution 1/40.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting