• Product NamePHGDH Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total PHGDH protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from internal residues of human phosphoglycerate dehydrogenase
• Target NamePHGDH
• Alternative Names    PDG; PGD; PGAD; PGDH; SERA; 3PGDH; 3-PGDH; HEL-S-113
• Accession No. Swiss-Prot#: O43175
  NCBI Gene ID: 26227
  Gene Accssion: NP_006614
• UniprotO43175
• Gene ID26227;
• SDS-PAGE MW 57kd
• Concentration 2.3mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  

This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known.

• Gel: 8%SDS-PAGE
  Lysates (from left to right): NIH/3T3 and A431 cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/400 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 1 minute

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting