• Product NamePTPN12 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total PTPN12 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to residues near the C terminal of human protein tyrosine phosphatase, non-receptor type 12
• Target NamePTPN12
• Alternative Names    PTPG1; PTP-PEST
• Accession No. Swiss-Prot#: Q05209
  NCBI Gene ID: 5782
  Gene Accssion: NP_001124480/Q05209
• UniprotQ05209
• Gene ID5782;
• SDS-PAGE MW 88kd
• Concentration 2.9mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:100-1:300

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains a C-terminal PEST motif, which serves as a protein-protein interaction domain, and may regulate protein intracellular half-life. This PTP was found to bind and dephosphorylate the product of the oncogene c-ABL and thus may play a role in oncogenesis.?

• Gel: 6%SDS-PAGE
  Lysates (from left to right): 231 and K562 cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/200 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 10 minutes
• Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue using #37849 at dilution 1/45.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting