• Product NamePUS10 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total PUS10 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from internal residues of human pseudouridylate synthase 10
• Target NamePUS10
• Alternative Names    DOBI; CCDC139
• Accession No. Swiss-Prot#: Q3MIT2
  NCBI Gene ID: 150962
  Gene Accssion: NP_653310/Q3MIT2
• UniprotQ3MIT2
• Gene ID150962;
• SDS-PAGE MW 60kd
• Concentration 2.8mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:50-1:200

Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs.

• Gel: 8%SDS-PAGE
  Lysates (from left to right): Mouse intestinum tenue tissue
  Amount of lysate: 40ug per lane
  Primary antibody: 1/200 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 3 seconds
• Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using #37852 at dilution 1/45.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting