• Product NameKCNJ15 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB,IHC
• Species ReactivityHuman, Mouse, Rat
• SpecificityThe antibody detects endogenous levels of total KCNJ15 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from internal residues of human potassium inwardly-rectifying channel, subfamily J, member 15
• Target NameKCNJ15
• Alternative Names    IRKK; KIR1.3; KIR4.2
• Accession No. Swiss-Prot#: Q99712
  NCBI Gene ID: 3772
  Gene Accssion: NP_001263364/Q99712
• UniprotQ99712
• Gene ID3772;
• SDS-PAGE MW 43kd
• Concentration 1 mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:50-1:200

Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein has a greater tendency to allow potassium to flow into a cell rather than out of a cell. Eight transcript variants encoding the same protein have been found for this gene.?

• Gel: 8%SDS-PAGE
  Lysates (from left to right): Mouse pancreas tissue
  Amount of lysate: 40ug per lane
  Primary antibody: 1/200 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 30 seconds
• Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using #37871 at dilution 1/35.
• Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-2: 231 and A549 cell lysates Primary antibody: KCNJ15 Antibody at dilution 1/500 Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution Exposure time: 40 seconds
• The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using KCNJ15 Antibody at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting