• Product NamePARP Mouse Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• PurificationAffinity purification using immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityAntibody detects endogenous PARP protein.
• Target NamePARP
• Alternative Names    COBA2; COL11A2; Collagen alpha-2(XI) chain; collagen, type XI, alpha 2; DFNA13
• Accession No. Swiss-Prot#:P13942
• UniprotP13942
• Gene ID1302;
• SDS-PAGE MW 116kd
• Concentration 1.0mg/ml
• Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:1000~1:3000

Poly [ADP-ribose] polymerase 1 (PARP-1) also known as NAD+ ADP-ribosyltransferase 1 or poly[ADP-ribose] synthase 1 is an enzyme that in humans is encoded by the PARP1 gene. PARP1 has a role in repair of single-stranded DNA (ssDNA) breaks. Knocking down intracellular PARP1 levels with siRNA or inhibiting PARP1 activity with small molecules reduces repair of ssDNA breaks. In the absence of PARP1, when these breaks are encountered during DNA replication, the replication fork stalls, and double-strand DNA (dsDNA) breaks accumulate.

• Western blot analysis of 1) Hela, 2) 293T, 3) Jurkat, using #38067 diluted at 1:2,000.
• Western blotting analysis using PARP Mouse Monoclonal Antibody #38067.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting