• Product NameTransferrin Mouse Monoclonal Antibody(7F4)
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.7F4
• PurificationAffinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHu
• SpecificityThe Transferrin Mouse Monoclonal Antibody detects endogenous Human Transferrin protein.
• Target NameTransferrin
• Alternative Names    Apotransferrin antibody, beta 1 metal binding globulin antibody, serotransferrin antibody, TF antibody
• Accession No. Swiss-Prot#:P02787
• UniprotP02787
• Gene ID7018;
• SDS-PAGE MW 77kd
• Concentration 1.0mg/ml
• Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB dilution: 1:1000~1:2000
  IHC dilution:1:50-300
  IF dilution:1:200

Transferrins are iron-binding blood plasma glycoproteins that control the level of free iron in biological fluids. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization.

• Western blot analysis of Human serum, using #38093 diluted at 1:2,000.
• Immunofluorescence analysis of Human-lung-cancer tissue. 1,Transferrin Monoclonal Antibody(7F4)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
• Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,Transferrin Monoclonal Antibody(7F4) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting