• Product NameVimentin Mouse Monoclonal Antibody(1A7)
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.1A7
• PurificationAffinity purification using immunogen.
• ApplicationsWB
• Species ReactivityHu Rt Ms
• SpecificityAntibody detects endogenous Vimentin protein.
• Target NameVimentin
• Alternative Names    FLJ36605 antibody, VIM antibody, VIME antibody
• Accession No. Swiss-Prot#:P08670
• UniprotP08670
• Gene ID7431;
• SDS-PAGE MW 50-57kd
• Concentration 1.0mg/ml
• Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB dilution: 1:1000~1:3000
  IF dilution: 1:100-200
  
  IF dilution:

The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules.Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells.

• Western blot analysis of 1) Hela, 2)Mouse Brain Tissue , 3) Rat Brain tissue, using #38097 diluted at 1:2,000.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting