• Product NameGalectin-3 Mouse Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.6G2
• PurificationAffinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHu
• SpecificityGalectin-3 Mouse monoclonal antibody detects endogenous Galectin-3 proteins..
• Target NameGalectin-3
• Alternative Names    Carbohydrate-binding protein 35; CBP 35; CBP35; Gal-3;
• Accession No. Swiss-Prot#:P17931
• UniprotP17931
• Gene ID3958;
• SDS-PAGE MW 26kd
• Concentration 1.0mg/ml
• Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB dilution: 1:1000~1:3000
  IHC dilution: 1:200
  
  IF dilution:1:100-200

Galectin 3 is one of the more extensively studied members of this family and is a 30 kDa protein. Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homooligomerized. Galectin 3 is normally distributed in epithelia of many organs, in various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins.

• Western blot analysis of Hela cell lysate, using #37995 diluted at 1:3,000.
• IHC staining of paraffin-embedded Human thyroid tissue with Galectin-3 mouse mAb£¨6G2£©diluted at 1:200.
• Immunofluorescence analysis of Human-lung-cancer tissue. 1,Galectin-3 Monoclonal Antibody(6G2)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting