• Product NameAMACR/P504S Mouse Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.4A12
• PurificationAffinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHu
• SpecificityAMACR/P504S Mouse Monoclonal antibody detects endogenous AMCAR/P504S proteins.
• Target NameAMACR/P504S
• Alternative Names    2-methylacyl-CoA racemase; Alpha-methylacyl-CoA racemase; AMACR; CBAS4; RACE
• Accession No. Swiss-Prot#:Q9UHK6
• UniprotQ9UHK6
• Gene ID23600;
• SDS-PAGE MW 42kd
• Concentration 1.0mg/ml
• Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB dilution: 1:1000~1:2000
  IHC dilution: 1:200
  
  IF dilution:1:200

AMACR has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. Expression of AMACR protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia.

• Western blot analysis of HepG2 cell lysate (lane 1) and mouse kidney (lane 2), using #37999 diluted at 1:1,000.
• IHC staining of mouse prostate adenocarcinoma tissue with AMACR/P504S mouse mAb(4A12) diluted at 1:200.
• Immunofluorescence analysis of Mouse-kidney tissue. 1,AMACR Monoclonal Antibody(4A12)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
• Immunofluorescence analysis of Rat-brain tissue. 1,AMACR Monoclonal Antibody(4A12)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
• Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1,AMACR Monoclonal Antibody(4A12) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
• Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,AMACR Monoclonal Antibody(4A12) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
• Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,AMACR Monoclonal Antibody(4A12) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting