• Product Namep53 Mouse Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.6C4
• PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHu
• Specificityp53 Mouse monoclonal antibody detects endogenous p53 proteins.
• Immunogen DescriptionSynthetic peptide of human p53
• Target Namep53
• Alternative Names    Tumor suppressor p53; Phosphoprotein p53; Antigen NY-CO-13
• Accession No. Swiss-Prot#:P04637
• UniprotP04637
• Gene ID7157;
• SDS-PAGE MW 53kd
• Concentration 1.0mg/ml
• Formulation PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
• Storage Store at -20˚C

• WB dilution: 1:2000
  IHC dilution: 1:200
  
  IF dilution:1:100-200

p53 plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro.

• IHC staining of paraffin-embedded Human colon cancer tissue with p53 mouse mAb(6C4) diluted at 1:200.
• IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.
• Figure legend: Western blot showing p53 (protein code: Tp53) expression in wild-type (WT) and Tp53 shRNA knockdown rat PC-12 cells. Beta-actin (Actb) served as the loading control. Conclusion: Since this antibody detects endogenous p53 protein in WT but not in shRNA knockdown cell lysate, this antibody is highly specific.
• Western blot analysis of lysates from 1) 293T, 2) HEK293, 3) A431 cells using p53 Mouse Monoclonal Antibody #38007 (Green) and Actin β Polyclonal Antibody as the loading control (Red).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting