• Product NameCD21 Mouse Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.2C5
• PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
• ApplicationsIHC,IF
• Species ReactivityHu Ms Rt
• SpecificityThe CD21 antibody detects endogenous CD21 proteins.
• Target NameCD21
• Alternative Names    C3DR; CD21; Complement C3d receptor; complement component (3d/Epstein Barr virus) receptor 2
• Accession No. Swiss-Prot#:P20023
• UniprotP20023
• Gene ID1380;
• SDS-PAGE MW 113kd
• Concentration 1.0mg/ml
• Formulation PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
• Storage Store at -20˚C

• IHC dilution: 1:200
  
  IF dilution:1:50-200

This gene encodes a membrane protein, which functions as a receptor for Epstein-Barr virus (EBV) binding on B and T lymphocytes. Genetic variations in this gene are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009],

• IHC staining of human tonsil tissue with CD21 mouse mAb(2C5) diluted at 1:200.
• Immunofluorescence analysis of Mouse-liver tissue. 1,CD21 Monoclonal Antibody(2C5)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
• Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1,CD21 Monoclonal Antibody(2C5) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
• Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,CD21 Monoclonal Antibody(2C5) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
• Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,CD21 Monoclonal Antibody(2C5) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting