• Product NameAquaporin 4 Mouse Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• PurificationAffinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHu Rt Ms
• SpecificityThe Aquaporin 4 Mouse Monoclonal antibody detects endogenous Aquaporin 4 proteins.
• Target NameAquaporin 4
• Alternative Names    AQP-4; AQP4; aquaporin 4; aquaporin type4; Aquaporin-4; HMIWC2
• Accession No. Swiss-Prot#:P55087
• UniprotP55087
• Gene ID361;
• SDS-PAGE MW 26kd
• Concentration 1.0mg/ml
• Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB dilution: 1:1000~1:2000
  IF dilution: 1:100-200
  IHC dilution:1:50-300

Aquaporin 4 belongs to the Aquaporin family of integral membrane proteins that conduct water through the cell membrane.

• Western blot analysis of 1) Hela, 2)Mouse Heart tissue , 3) Rat Heart Tissue, using #38017 diluted at 1:2,000
• IF analysis of Hela with #38017(Left) and DAPI (Right) diluted at 1:100.
• Immunofluorescence analysis of Human-appendix tissue. 1,Aquaporin 4 Monoclonal Antibody(4H1)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
• Immunofluorescence analysis of Mouse-brain tissue. 1,Aquaporin 4 Monoclonal Antibody(4H1)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
• Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,Aquaporin 4 Monoclonal Antibody(4H1) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting